MODEL ORGANISMS
Our choice of model organisms for systems biotechnology purpose is dictated by the industrial usefulness and scientific interest. We are systematically combining and integrating the results of multi-omics analyses of the genome, transcriptome, proteome, and phenome of the strain to better understand biological systems and the underlying biological networks. We are constructing in silico genome-scale metabolic and regulatory network model of the select organisms, which predictive power will be critical to develop customized genome and cost-effective production of bio-products.
Escherichia coli
A primary goal of the bioprocess development is the cost-effective production of desired products such as therapeutic and industrial proteins on a large scale. E. coli strains are preferred production hosts because of fast growth, facility in genetic modification and cultivation, and high yields for many recombinant proteins.
• Escherichia coli K-12 MG1655
Streptomyces species
Streptomyces are fascinating soil bacteria of major economic and medical importance. As with the other Actinobacteria, Streptomyces are gram-positive, and have large genomes with high GC-content. They produce about 70% of antibiotics of natural origin and other secondary metabolites for treatment of, for example, cancer, a range of infections, high cholesterol, or have immunosuppressive activity. The ability to synthesize high-valued bioactive compounds has driven a great deal of research into using Streptomyces species as an industrial production host. However, complexity of secondary metabolism and its regulation makes it difficult to design and re-engineer Streptomyces as cell factories.
• Streptomyces coelicolor A3(2) strain M145
• Streptomyces venezuelae ATCC 10712
• Streptomyces venezuelae ATCC 15439 (Genome sequencing project was funded by Intelligent Synthetic Biology Center)
Pseudomonas putida
Methanotroph (메탄자화균)
• Methylosinus trichosporium OB3b