LOCUS       CP001280               10891 bp    DNA              UNK 01-JAN-1980
DEFINITION  Methylocella silvestris BL2, complete genome.
ACCESSION   CP001280
VERSION     CP001280.1
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     cluster         1..10891
                     /note="Cluster number: 2"
                     /note="Detection rule(s) for this cluster type:
                     bacteriocin: (strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or
                     Bacteriocin_Iii or Cloacin or Linocin_M18 or TIGR03603 or
                     TIGR03604 or TIGR03605 or TIGR03651 or TIGR03678 or
                     TIGR03693 or TIGR03798 or TIGR03882 or TIGR03601 or
                     TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or
                     DUF692);"
                     /clusterblast="2. CCJP01000005_c1	Polaromonas sp. CG9_12
                     genome assembly Polaromonas sp stra... (17% of genes show
                     similarity)"
                     /clusterblast="3. NZ_ARWB01000001_c5	Methylopila sp. M107
                     A3OUDRAFT_chromosome1.1_C, whole g... (15% of genes show
                     similarity)"
                     /clusterblast="4. NZ_ARWA01000001_c6	Methyloferula stellata
                     AR4 strain AR4T A3OQDRAFT_chromo... (13% of genes show
                     similarity)"
                     /clusterblast="5. CP004015_c4	Rhizobium tropici CIAT 899,
                     complete genome. (15% of genes show similarity)"
                     /clusterblast="6. AEYE02000002_c1	Rhizobium grahamii CCGE
                     502 contig0002, whole genome shotg... (15% of genes show
                     similarity)"
                     /clusterblast="7. AP009384_c7	Azorhizobium caulinodans ORS
                     571 DNA, complete genome. (13% of genes show similarity)"
                     /clusterblast="8. AKKA01000087_c1	Rhizobium sp. CF122
                     PMI09_contig154.154, whole genome shot... (13% of genes
                     show similarity)"
                     /clusterblast="9. AQRC01000006_c1	Thioclava sp. 13D2W-2
                     contig6, whole genome shotgun sequen... (13% of genes show
                     similarity)"
                     /clusterblast="10. CP003563_c6	Sinorhizobium fredii USDA
                     257, complete genome. (13% of genes show similarity)"
                     /clusterblast="11. APMP01000003_c1	Caulobacter crescentus
                     OR37 OR37_contig00003, whole genom... (13% of genes show
                     similarity)"
                     /product="bacteriocin"
     gene            152..1000
                     /locus_tag="Msil_0297"
     CDS             152..1000
                     /locus_tag="Msil_0297"
                     /codon_start=1
                     /product="nicotinate-nucleotide pyrophosphorylase"
                     /EC_number="2.4.2.19"
                     /note="KEGG: nwi:Nwi_2426 nicotinate-nucleotide
                     pyrophosphorylase; TIGRFAM: nicotinate-nucleotide
                     pyrophosphorylase; PFAM: Quinolinate phosphoribosyl
                     transferase"
                     /db_xref="GI:217501867"
                     /db_xref="InterPro:IPR002638"
                     /db_xref="InterPro:IPR004393"
                     /translation="MLAPLPRLLTEPLVRGALLEDLGRAGDVTSDAIAPASLNMRAVLA
                     ARQPGVVAGLDLARLAFELIDPRIFVRIERPDGAAVAAGDRIAVITGPARGVLTAERTA
                     LNFLSHLSGVATATATLVAAVKGYKAKIVCTRKTTPGLRAVEKYAVRAGGGSNHRFGLD
                     DALLIKDNHVAIAGGVAEALRRAKAAAGHLVKIELEVDTLQQLVEAMEQGVDVVLLDNM
                     TPDMLTEAVRIVAGRAVTEASGRITAASAPAVAASGVDVISVGWLTHSAPILDIGLDFE
                     P"
                     /transl_table=11
                     /protein_id="ACK49276.1"
     gene            complement(1021..1317)
                     /locus_tag="Msil_0298"
     CDS             complement(1021..1317)
                     /locus_tag="Msil_0298"
                     /codon_start=1
                     /product="hypothetical protein"
                     /transl_table=11
                     /db_xref="GI:217501868"
                     /translation="MSAGIVELAPCFTLENAMKKGLFAAVAFSALALTAGAASAQIEFP
                     LLIADVQYSQPMLGVTPIETVQEGRSAFAPAPADTLTPFDEFAPIDFELEPAQ"
                     /protein_id="ACK49277.1"
     sig_peptide     complement(1195..1317)
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 0.977) with cleavage site probability 0.868 at
                     residue 41"
                     /locus_tag="Msil_0298"
     gene            1505..2323
                     /locus_tag="Msil_0299"
     CDS             1505..2323
                     /locus_tag="Msil_0299"
                     /codon_start=1
                     /product="Rhodanese domain protein"
                     /transl_table=11
                     /note="PFAM: Rhodanese domain protein; KEGG: mlo:mlr5406
                     hypothetical protein"
                     /note="smCOG: SMCOG1163:rhodanese_domain-containing_protein
                     (Score: 34.4; E-value: 3e-10);"
                     /note="smCOG tree PNG image: smcogs/Msil_0299.png"
                     /db_xref="GI:217501869"
                     /db_xref="InterPro:IPR001763"
                     /translation="MPSNTEITPSQLTRLVGLSTAPTIVDLRTEEEFASDPRLLPASRR
                     RDARMVEILAKELIGRDVVVLCQRGSDPSQGAAAWLRQAGVKAETLEGGFDAWRTGGGL
                     LVKTAKLPPADAAGRTLWVTRARPKVDRIACPWLIRRFVDPAAVFLFVAASEVGAVAER
                     FNGAAFDIDNVFWSHRGERCAFDTMIEEFGLKSEPLARLATIVRAADTARLDLAPEAPG
                     FLAASLGLSRMFRDDLEQLEAGLLLYDAFYRWSRDATDETHNWPPGRKMA"
                     /protein_id="ACK49278.1"
     gene            2335..3738
                     /locus_tag="Msil_0300"
     CDS             2335..3738
                     /locus_tag="Msil_0300"
                     /codon_start=1
                     /product="chromate transporter, chromate ion transporter
                     (CHR) family"
                     /transl_table=11
                     /note="TIGRFAM: chromate transporter, chromate ion
                     transporter (CHR) family; PFAM: Chromate transporter; KEGG:
                     mlo:mlr5407 chromate transporter"
                     /db_xref="GI:217501870"
                     /db_xref="InterPro:IPR003370"
                     /db_xref="InterPro:IPR014047"
                     /translation="MSVNFRPQETMAPPAPPTFAEAARVWAKIGLLSFGGPAGQIALMH
                     KELVEERRWIGEQRFLHALNYCMVLPGPEAQQLAVYIGWLMHRAPGGLVAGVLFVLPGA
                     VVMLALSIIYALTRTAPVVDALFFGVKAAVLAVVVEAMLRIARRALKNRAMVAIAAAAF
                     LGLYVFHIPFPLIILAAGALGWIGARYAPELFIARGHGSAGSAPDLKGVVDGMFERGEM
                     AHTEPSLARALRVLAVWLPIWLGPVAALWILTGWSSVWTQIGAFFSSMAVVTFGGAYAV
                     LAYVAQAAVVAFGWLAPGEMVDGLGLAETTPGPLVMVLQFVGFLAAYRAPGALTPLLAG
                     ALGALLTSWVTFAPCFLWIFLGGPYIEALRDNKGISAALSAITAAVVGVIMNLALWFAL
                     HVIFREAWRFDFLGISPDLPVIASIDWRAALLSMAALVAMLRFKIGMAPTLLACAAGGA
                     VLLKLTAAG"
                     /protein_id="ACK49279.1"
     gene            complement(3744..4217)
                     /locus_tag="Msil_0301"
     CDS             complement(3744..4217)
                     /locus_tag="Msil_0301"
                     /codon_start=1
                     /product="DoxX family protein"
                     /transl_table=11
                     /note="PFAM: DoxX family protein; KEGG: bid:Bind_3128 DoxX
                     family protein"
                     /db_xref="GI:217501871"
                     /db_xref="InterPro:IPR011637"
                     /translation="MDITHRISEPAVPVRAIQIFIDLLNRIPQSFLSLCARVFPAAVFW
                     MSGETKVDGWHLSDSAVDLFRDEYRLPLIDPSIAAHLSAFAEHFFPFLLVVGLASRFAA
                     LALLGMTLVIEIFVYPDAWPTHGVWATCFLVVIARGPGVFSLDHLIARRYGAK"
                     /protein_id="ACK49280.1"
     gene            complement(4201..5004)
                     /locus_tag="Msil_0302"
     CDS             complement(4201..5004)
                     /locus_tag="Msil_0302"
                     /codon_start=1
                     /product="conserved hypothetical protein"
                     /transl_table=11
                     /note="KEGG: azc:AZC_3486 hypothetical protein"
                     /db_xref="GI:217501872"
                     /translation="MTSVMSSLAGDFAAALRDPLATLPAALSAHAAPAASKRFAVYRNN
                     VAVSLIGALEARFPATRRLVGDEFFRAMACLYIKAQPPRSPLMMRYGDELPGFIASFEP
                     AAEIVYLADIARLEAARTQAYHAADAEPLELSALAALDPESLARLRLAPHPSLDIVESA
                     FPIVTIWASCTGEAPLTAIEDWRCETALIARPRLEVEVRRAPPGAAAFLEALGRGMSFG
                     EAANSAAARAKDFDLTLMIASLIGGGLVVSADGAKRMETIHGYHA"
                     /protein_id="ACK49281.1"
     gene            complement(5001..5891)
                     /locus_tag="Msil_0303"
     CDS             complement(5001..5891)
                     /locus_tag="Msil_0303"
                     /codon_start=1
                     /product="protein of unknown function DUF692"
                     /transl_table=11
                     /note="PFAM: protein of unknown function DUF692; KEGG:
                     azc:AZC_3485 hypothetical protein"
                     /db_xref="GI:217501873"
                     /db_xref="InterPro:IPR007801"
                     /sec_met="Type: bacteriocin"
                     /sec_met="Domains detected: DUF692 (E-value: 1.1e-83,
                     bitscore: 278.3, seeds: 7)"
                     /sec_met="Kind: biosynthetic"
                     /translation="MNYISAPPALLPARAGVGFKPEHFDGIAAERQPLGFFEVHAENYM
                     GAGGVPHAQLRFLRERYALSLHGVGLSIGSPEPLDRDHLSRLRRLCALYQPESFSEHLA
                     WSSHGGVYFNDLLPLPYTEETLAGVAAHVDETQTALGGRILIENPATYVRFSQSDIPET
                     EFLAELARRTGCGLLLDLNNVFVSARNHGESALGYLASFPLRSVGEIHLAGFFDASEEA
                     AAPLLIDSHGATVADEVVSLFEWTIEQTGPLPTLIEWDNDVPDWPVLLAEAMKAQDRLR
                     HAERGYARRLDRRAL"
                     /protein_id="ACK49282.1"
     gene            complement(5949..6245)
                     /locus_tag="Msil_0304"
     CDS             complement(5949..6245)
                     /locus_tag="Msil_0304"
                     /codon_start=1
                     /product="conserved hypothetical protein"
                     /transl_table=11
                     /note="KEGG: bid:Bind_3131 hypothetical protein"
                     /db_xref="GI:217501874"
                     /translation="MSANTSLTASALAGAFAAALSLIASQASAGPVEPQPGKDKCYGIS
                     LKGHNDCAAGAGTSCAGTSKVDYDGGAWKYVAKGACDTIKTPHGTGSLAPTKS"
                     /protein_id="ACK49283.1"
     sig_peptide     complement(6156..6245)
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.973 at
                     residue 30"
                     /locus_tag="Msil_0304"
     gene            6440..6997
                     /locus_tag="Msil_0305"
     CDS             6440..6997
                     /locus_tag="Msil_0305"
                     /codon_start=1
                     /product="RNA polymerase, sigma-24 subunit, ECF subfamily"
                     /transl_table=11
                     /note="TIGRFAM: RNA polymerase sigma factor, sigma-70
                     family; PFAM: sigma-70 region 2 domain protein; Sigma-70
                     region 4 type 2; KEGG: rpb:RPB_3555 RNA polymerase sigma
                     factor"
                     /note="smCOG:
                     SMCOG1032:RNA_polymerase,_sigma-24_subunit,_ECF_subfamily
                     (Score: 102.5; E-value: 3.3e-31);"
                     /note="smCOG tree PNG image: smcogs/Msil_0305.png"
                     /db_xref="GI:217501875"
                     /db_xref="InterPro:IPR007627"
                     /db_xref="InterPro:IPR013249"
                     /db_xref="InterPro:IPR014284"
                     /translation="MRVEDREKQWRLLMLSANGGDAAAYRNLLVQLTPALRAFARRGVA
                     RAGAALTDAEDIVQETLLAVHLKRQTWDEQAPLSPWLFAIARHKLIDALRRRGRRIELS
                     IDDFAESLPSADAGSDSIVSDVKRHLEELPPGQRKVVNCIAVEGVSIDEAASRLSMSNG
                     AVRVALHRGLAALAAKFRSVES"
                     /protein_id="ACK49284.1"
     gene            6994..7626
                     /locus_tag="Msil_0306"
     CDS             6994..7626
                     /locus_tag="Msil_0306"
                     /codon_start=1
                     /product="protein of unknown function DUF1109"
                     /transl_table=11
                     /note="PFAM: protein of unknown function DUF1109; KEGG:
                     rpe:RPE_1945 protein of unknown function DUF1109"
                     /db_xref="GI:217501876"
                     /db_xref="InterPro:IPR009495"
                     /translation="MKTSELINAISEDREMPLAPGRALCLALIPGLAVAIALFSRFGPR
                     PHFLSLLADPRFSLKLLVCYLLAALAGLFVLRLFRPGGSWRGALFLLALPPALLGVGVI
                     FELFVVSPANWETTMIGHNAMFCLKTIPLLGIAPLIAILVALRNGAPERPAVAGAGAGL
                     FAGAIGAALYASHCPDDSPLFVAVWYPIAIAFLTALGAAVGARLLRW"
                     /protein_id="ACK49285.1"
     gene            complement(7631..9346)
                     /locus_tag="Msil_0307"
     CDS             complement(7631..9346)
                     /locus_tag="Msil_0307"
                     /codon_start=1
                     /product="AMP-dependent synthetase and ligase"
                     /transl_table=11
                     /note="PFAM: AMP-dependent synthetase and ligase; KEGG:
                     bid:Bind_3436 AMP-dependent synthetase and ligase"
                     /note="smCOG: SMCOG1002:AMP-dependent_synthetase_and_ligase
                     (Score: 378.6; E-value: 6.3e-115);"
                     /db_xref="GI:217501877"
                     /db_xref="InterPro:IPR000873"
                     /sec_met="Type: other"
                     /sec_met="Domains detected: AMP-binding (E-value: 2.2e-96,
                     bitscore: 320.8, seeds: 400)"
                     /sec_met="Kind: biosynthetic"
                     /sec_met="NRPS/PKS subtype: NRPS-like protein"
                     /sec_met="NRPS/PKS Domain: AMP-binding (61-501). E-value:
                     2.6e-98. Score: 320.8; NRPS/PKS Domain: Msil_0307_A1;
                     Substrate specificity predictions: N/A (NRPSPredictor2
                     SVM), leu (Stachelhaus code), glu (Minowa), nrp
                     (consensus);"
                     /translation="MREQAVDLRPPWLKTYPSSVPAEIETPRYRLLAEAPADLAARRGG
                     ASAFTTVMPNGMSGRLSFNDVDRLSDAFAAYLRENLKLPTGARIAVQTPNGLAYPIVAF
                     GIFKAGCTLINVNPLYTATEMMHLFADAQPDAIVAIDMFAEKLADALENAPIPNVVLTS
                     AASLFPAAPRLIIGAVQKYVRKEIPRPRFAACAFAQALAMGAQKISEGADVRAYTAGLD
                     PDAVACLQYTGGTTGVSKAAMLSHRNLIVNVAQFLSFVGGAIREDDHVLTALPLYHCFA
                     FTVNMLGFFFRGAHNVLIPNPRPLVNLRPAFAKAPISFITGVNTLFKGLLHERWFVEKP
                     PQHLRLSVAGGMALHESVAKRWEQVTQTPLIEGYGLSEASPVLTFNPTLRVKPGTIGVP
                     LPWTDIKCVDDNGREVAPGEHGELIARGPQIMSGYWRQPEETRIALRDGWLYTGDIATM
                     DEEGYFTLVDRRKDMILVNGFNVYPNEVEATLTEHPGVKECAVVGVPDAGVGEAVRAFI
                     VRADKSLDAEELRDFCRTRLAPYKIPRHVVFRDDLPKSNVGKILRKDLRGDMSP"
                     /protein_id="ACK49286.1"
     aSDomain        complement(7844..9163)
                     /domain="AMP-binding"
                     /database="nrpspksdomains.hmm"
                     /evalue="2.60E-98"
                     /specificity="NRPSpredictor2 SVM: N/A"
                     /specificity="Stachelhaus code: leu"
                     /specificity="Minowa: glu"
                     /specificity="consensus: nrp"
                     /locus_tag="Msil_0307"
                     /label="Msil_0307_A1"
                     /detection="hmmscan"
                     /score="320.8"
                     /aSASF_note="ASF analyisis with definition
                     StachelhausExtract (type active_site)"
                     /aSASF_scaffold="Scaffold coordinates:
                     (47,48,51,93,125,127,154,162,163); scaffold residues:
                     (A,F,N,T,V,G,G,P,V); expected: (D,A,W,T,I,A,A,I,C);
                     matchArray:
                     (False,False,False,True,False,False,False,False,False);
                     emission probability array
                     (n.d.,n.d.,n.d.,n.d.,n.d.,n.d.,n.d.,n.d.,n.d.); overall
                     match: FALSE"
                     /translation="SFNDVDRLSDAFAAYLRENLKLPTGARIAVQTPNGLAYPIVAFGI
                     FKAGCTLINVNPLYTATEMMHLFADAQPDAIVAIDMFAEKLADALENAPIPNVVLTSAA
                     SLFPAAPRLIIGAVQKYVRKEIPRPRFAACAFAQALAMGAQKISEGADVRAYTAGLDPD
                     AVACLQYTGGTTGVSKAAMLSHRNLIVNVAQFLSFVGGAIREDDHVLTALPLYHCFAFT
                     VNMLGFFFRGAHNVLIPNPRPLVNLRPAFAKAPISFITGVNTLFKGLLHERWFVEKPPQ
                     HLRLSVAGGMALHESVAKRWEQVTQTPLIEGYGLSEASPVLTFNPTLRVKPGTIGVPLP
                     WTDIKCVDDNGREVAPGEHGELIARGPQIMSGYWRQPEETRIALRDGWLYTGDIATMDE
                     EGYFTLVDRRKDMILVNGFNVYPNEVEATLTEHPGVKECAV"
                     /asDomain_id="nrpspksdomains_Msil_0307_A1"
     CDS_motif       complement(7886..7945)
                     /detection="hmmscan"
                     /locus_tag="Msil_0307"
                     /motif="NRPS-A_a8"
                     /database="abmotifs"
                     /evalue="6.30E-04"
                     /asDomain_id="nrpspksmotif_Msil_0307_0003"
                     /note="NRPS/PKS Motif: NRPS-A_a8 (e-value: 0.00063,
                     bit-score: 12.6)"
                     /score="12.6"
                     /aSTool="pksnrpsmotif"
                     /translation="DRRKDMILVNGFNVYPNEVE"
                     /label="NRPS-A_a8"
     CDS_motif       complement(8024..8098)
                     /detection="hmmscan"
                     /locus_tag="Msil_0307"
                     /motif="NRPS-A_a6"
                     /database="abmotifs"
                     /evalue="1.70E-06"
                     /asDomain_id="nrpspksmotif_Msil_0307_0002"
                     /note="NRPS/PKS Motif: NRPS-A_a6 (e-value: 1.7e-06,
                     bit-score: 20.4)"
                     /score="20.4"
                     /aSTool="pksnrpsmotif"
                     /translation="PGEHGELIARGPQIMSGYWRQPEET"
                     /label="NRPS-A_a6"
     CDS_motif       complement(8609..8668)
                     /detection="hmmscan"
                     /locus_tag="Msil_0307"
                     /motif="NRPS-A_a3"
                     /database="abmotifs"
                     /evalue="5.70E-05"
                     /asDomain_id="nrpspksmotif_Msil_0307_0001"
                     /note="NRPS/PKS Motif: NRPS-A_a3 (e-value: 5.7e-05,
                     bit-score: 15.9)"
                     /score="15.9"
                     /aSTool="pksnrpsmotif"
                     /translation="ACLQYTGGTTGVSKAAMLSH"
                     /label="NRPS-A_a3"
     gene            9535..9813
                     /locus_tag="Msil_0308"
     CDS             9535..9813
                     /locus_tag="Msil_0308"
                     /codon_start=1
                     /product="conserved hypothetical protein"
                     /transl_table=11
                     /note="KEGG: bid:Bind_3437 hypothetical protein"
                     /db_xref="GI:217501878"
                     /translation="MAQIPAQNSPSPANLATPKPINWLHLSTIVAVAILVGTEMVGASW
                     AAGWAIGGLLQLDPSISRLIEIGFTLCGFVLLYFFMRIAIRHEPIRG"
                     /protein_id="ACK49287.1"
     sig_peptide     9869..9952
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probability 1.000) with cleavage site probability 0.577 at
                     residue 28"
                     /locus_tag="Msil_0309"
ORIGIN
        1 cccatgcccg cacggattat cccggtcttg cggcggcgcc ccgccgaacc acgctacggc
       61 ttgacgaagc ggtgcgggcg gccgaggaat tccttcccga aatggccgac tgaaattcgt
      121 ccaggacatg gtgtgagatg agccccgcgc gatgctagcg ccgctgccaa gattgctgac
      181 cgagccgctg gttcgcggcg cgctgctgga agatcttggc cgggcgggcg atgtcacctc
      241 cgacgccatc gctccggcca gcctcaacat gcgcgcggtt ctcgccgccc ggcagccggg
      301 agtggtggct ggcctcgacc ttgcgcggct ggctttcgaa ctgatcgatc cgcgcatctt
      361 tgtccgcatc gagcgtccgg acggcgcagc cgtcgcggcg ggcgatcgga tcgctgtcat
      421 caccggaccc gcgcggggcg ttctgacagc cgagcggacg gccctgaact ttctttccca
      481 tctcagcggc gttgcgaccg caaccgccac gctggtcgcg gcggtcaaag ggtataaggc
      541 caagatcgtc tgcacgcgga aaaccacgcc cggcctgagg gcggtcgaga aatatgccgt
      601 gcgcgccggc ggtggctcga accatcggtt cggcctcgac gacgctctgc tgatcaagga
      661 caatcatgtc gccatcgccg gcggcgtcgc cgaggcgttg cggcgcgcga aagccgcagc
      721 cggccatctc gtcaagatcg agcttgaggt cgatacgctg caacagctgg tggaggcgat
      781 ggagcagggg gtcgacgtcg tcctgctcga caatatgact cccgacatgc tgacagaggc
      841 tgtcaggatc gtcgcgggga gggccgttac agaagcgtct gggaggataa cggcagcgtc
      901 ggcccccgcc gtcgcggcgt ccggggtcga tgtcatttcg gtgggctggc taacgcatag
      961 cgcgccaatc ctcgacatcg ggctcgattt cgagccctga gccgaagcgc cgcctgatgg
     1021 ttactgcgcg ggttccagct cgaaatcgat cggggcgaat tcgtcgaagg gggtcagcgt
     1081 gtcggcgggc gccggcgcga aagcgctacg gccttcctga accgtctcga tcggcgtcac
     1141 gccaagcatc ggctggctat attggacgtc ggcgataagg agcggaaatt caatctgggc
     1201 cgaggccgcg ccggctgtca gagcaagggc gctaaaagcg accgcggcga aaagaccttt
     1261 tttcatggcg ttctccagag tgaaacatgg cgctagctct acaatgccgg cggacatgaa
     1321 aggttccccg ctttccggcg aaaccgggga aaatcccgtg tgttcgcgcc gccatcaaaa
     1381 gcaagagtca ttgacgccaa aggagcggcc gtggttagtt gccgccatgg ggttgatcgc
     1441 gattaccccg tgaggcgcca gcccaaagat cgcgtccatg atcccttaaa agggttggac
     1501 gctgatgccc tcaaatacag aaattacccc ctcgcagcta actcggctcg tcggtttgtc
     1561 gaccgcgccg accatcgtcg atctccgcac ggaggaggag ttcgcctccg acccccgcct
     1621 gctgccggct tcccgccgcc gcgacgcgcg gatggtcgaa atccttgcca aagaactgat
     1681 tggccgcgac gtcgtcgtgc tctgtcagcg cggatctgat ccgagccagg gggccgccgc
     1741 ctggctgcgt caggccggcg tcaaggccga aacgctggaa ggcggttttg acgcctggcg
     1801 cacgggaggc ggcctgcttg tcaaaaccgc caagctgccg ccggccgacg ccgccgggcg
     1861 aacgctttgg gtgacgcgcg cgcggccgaa ggtcgaccgc atcgcctgcc cctggctgat
     1921 tcgccgcttt gtcgatccgg ccgccgtttt tctctttgtt gcggcctccg aggtcggcgc
     1981 cgtggcagaa cgcttcaacg gcgcggcttt cgacatcgac aatgtgttct ggagccatcg
     2041 cggcgagcga tgcgccttcg atacgatgat cgaggaattc ggcctgaaat ctgagccgct
     2101 cgcgcggctg gcgacgatcg tgcgggcggc cgataccgct cggctcgatc tcgcgccgga
     2161 agcgccgggc tttctcgccg cctcgctcgg actgtcgcgc atgttccgcg acgatctcga
     2221 gcagctcgag gcgggactgc ttctctatga cgccttctat cgctggtcgc gcgacgccac
     2281 cgacgagacg cataactggc cgcccggccg gaaaatggcg tgagggaggc gagcatgtcc
     2341 gtcaatttca ggccgcagga aacgatggct ccgcccgcgc ctccgacgtt tgcggaggcc
     2401 gcgagggtct gggccaaaat cggcctgctg agcttcggcg gcccggccgg ccagatcgcc
     2461 ttgatgcata aggagcttgt cgaagagcgc cgctggattg gcgagcagcg ctttctgcat
     2521 gctctcaact attgcatggt gttgccgggt cccgaggcgc agcagcttgc tgtctacatc
     2581 ggctggctga tgcatcgcgc gcccggcggc ctcgtcgccg gcgtgttgtt tgttctgccc
     2641 ggcgctgttg tgatgctcgc ccttagcatc atctacgcgc tcactcgcac cgcccctgtg
     2701 gtcgatgcgc tctttttcgg cgtgaaggcg gcggtgcttg ccgttgtcgt cgaggctatg
     2761 ctgcgaatcg cccgccgtgc gctgaagaat cgggcgatgg tcgcgatcgc cgccgccgcc
     2821 tttctcggtc tctatgtgtt tcatattccg ttcccgctga tcattttggc cgccggcgcc
     2881 ctgggctgga ttggcgcccg ttacgctccc gagctcttca tcgcccgcgg acatggttcg
     2941 gcgggttcgg cgccagatct caagggggtc gtcgacggca tgtttgagcg cggcgagatg
     3001 gctcacacgg agccttcgct cgcgcgcgct ttgcgcgtac tcgccgtctg gctgccgatc
     3061 tggcttggtc cggtcgcagc gctctggatt ctcactggat ggtccagcgt ctggacgcag
     3121 atcggcgcct ttttcagctc gatggcggtc gtgaccttcg gcggcgccta tgcggttctg
     3181 gcctatgtgg cgcaagcggc ggtcgtcgcc ttcggctggc ttgcgcctgg cgaaatggtc
     3241 gacggcctcg gtctcgccga gacgacgccg gggccgctgg tgatggtgct gcaatttgtc
     3301 ggcttcctcg ccgcctatcg cgcgccgggc gcgctgaccc ctctgctcgc tggggccctt
     3361 ggcgcgctgc tcaccagctg ggtgacgttc gcgccatgct ttctctggat ctttctcggc
     3421 ggcccctaca tcgaagcgct gcgcgacaat aaggggatct ccgccgccct gtcggccatc
     3481 acggcggccg tcgttggcgt gatcatgaat cttgcgttgt ggtttgcctt gcacgtcatc
     3541 tttcgtgagg cgtggcgctt cgattttctc gggatcagtc ccgacctgcc ggtgatcgct
     3601 tcgatcgatt ggcgcgccgc gcttctgtcc atggcggcgc tggtcgcgat gttgcgattc
     3661 aagattggca tggcgccgac gctcctcgcc tgcgcggccg gcggcgccgt gcttttgaag
     3721 ctgacggcgg cgggctgatc tttctatttg gccccgtagc gccgggcgat cagatgatcc
     3781 agcgaaaaaa cgcccgggcc gcgggcgatg accacaagaa agcatgtcgc ccacacgcca
     3841 tgtgtcggcc atgcgtcggg gtagacgaag atttcaatga caagcgtcat tccaagcaaa
     3901 gcgagcgcgg cgaagcgcga agcgaggccg acgacgagca ggaacggaaa gaaatgctcc
     3961 gcgaaggccg aaagatgcgc cgcgatcgac ggatcgatca ggggcagcct gtattcgtcg
     4021 cggaataaat cgacggcgct gtcgctaaga tgccagccat cgaccttggt ctcgccggac
     4081 atccagaaca cggcggcggg aaacacgcgc gcgcagagcg aaaggaagga ttgcggaatt
     4141 cggttgagaa ggtcgatgaa gatttggatc gcccgcacgg ggacggccgg ctcggaaatt
     4201 ctatgcgtga tatccatgaa ttgtctccat ccgtttggcg ccgtccgctg agacgacgag
     4261 cccgccgccg atcagactgg cgatcatgag agtgaggtcg aaatctttcg cgcgagcggc
     4321 ggcgctattg gcggcctcac cgaaactcat gccgcgccct aaagcttcga ggaacgccgc
     4381 tgcgccaggc ggagcgcggc ggacctcgac ttcaagccgc ggccgcgcga tgagggcggt
     4441 ttcgcagcgc caatcctcga tcgccgtcaa aggcgcttcg ccggtgcagc ttgcccagat
     4501 ggtgacgatc ggaaacgccg attcgacaat gtccagcgac ggatgcggcg cgaggcgcag
     4561 acgcgcgaga ctctcgggat cgagcgccgc gagggctgac aattcgagcg gctcggcgtc
     4621 ggcggcgtga taggcttgcg tgcgcgcggc ctcgaggcgc gcgatatcgg caagataaac
     4681 gatctcggcg gccggctcga aactcgcgat gaagcccggc aattcgtcgc catagcgcat
     4741 catcagcggc gaacgcggcg gctgcgcctt gatatagaga cacgccatgg cgcgaaaaaa
     4801 ttcatctccg acgagacggc gcgtcgccgg gaaacgcgcc tctagcgcgc cgatcaaact
     4861 caccgcgaca ttgttgcgat agacagcaaa gcgcttcgat gccgccggag cggcgtgagc
     4921 cgaaagcgca gcgggcagcg tggcgagcgg atcgcgcaac gcggcggcga aatcgcccgc
     4981 aaggctagac atgacggacg tcataacgct ctccggtcaa gtcgccgcgc atagccgcgt
     5041 tcggcatggc gaagccggtc ttgcgctttc atcgcctcgg caagcagcac gggccagtcc
     5101 ggaacgtcat tgtcccattc gatcagcgtc ggcagaggtc cggtttgttc gattgtccac
     5161 tcgaacaagg agacgacctc gtctgccacc gtcgccccat gcgaatcgat gagcaaaggc
     5221 gcggccgcct cctcgctggc gtcgaaaaat ccggcaagat gaatctcgcc cacgctgcgc
     5281 agcggaaagc tggcgagata gccgagcgcc gactcgccgt gattgcgcgc gctgacaaat
     5341 acattgttga gatcaagcag cagaccgcac cccgtgcgcc gtgcgagctc ggccaaaaat
     5401 tccgtttcgg gaatgtcgct ttgcgaaaag cggacgtagg tcgccggatt ttcgatgagg
     5461 atgcgcccac ccagcgcggt ctgcgtctcg tcgacgtggg cggcgacgcc ggcgagggtt
     5521 tcttccgtgt agggcagggg cagcaaatcg ttaaaataca cgccgccatg cgatgaccag
     5581 gcaagatgct ctgaaaagct ctccggctga tagagggcgc aaagccgcct caagcgggaa
     5641 aggtgatcgc gatcgagcgg ctcgggcgag ccgatcgaga gacccacccc atgcagcgaa
     5701 agcgcgtagc gctcgcgcag gaagcgcagc tgtgcatggg gaacgccccc cgcgcccatg
     5761 tagttttcgg catgaacctc gaagaagccg agcggctgcc gttctgcggc gatcccgtcg
     5821 aaatgctccg gcttgaagcc gacgccggcg cgcgccggaa gaagcgcagg cggggcggaa
     5881 atgtaattca tagcgcgctc caagcgatgc ggcgcgcgaa aactccgcgc gccggtcaat
     5941 ccctcttgtc aggatttggt gggagcgagc gaacccgttc catgcggcgt cttgatagtg
     6001 tcgcaggcgc ctttggcgac atatttccag gcgccgccgt cataatcgac cttggaggtg
     6061 ccggcgcagg aggttccggc gcccgccgcg caatcattgt ggcctttcag cgagatcccg
     6121 tagcatttgt ccttgcccgg ctgcggctcg accgggccgg ccgaggcctg cgacgcaata
     6181 agggacagag cggcggcaaa agcgccggcg agagcggaag cggtcaaact ggtgttggcg
     6241 gacatgttcg tttcctttat cgagagggac tgcccaggcg aagcatctgc gccttgcgcc
     6301 ttcatcttcg cctctacggc gcggggcgtt acacgggacg tcgctttttt ggcgttgacg
     6361 aattattgac cgttctttga cgcagtcgtg taacgccagc ggcgacggcg gcgaagatca
     6421 tcatgcgatt ggagcgcgcg tgcgcgtcga ggaccgcgaa aagcaatggc gcctgctcat
     6481 gctgtcggcc aatgggggcg acgccgcggc ctatcggaat ttgcttgtcc agctgacgcc
     6541 ggcgctgcgc gccttcgcgc gaaggggcgt cgcgcgcgca ggcgccgcgc tgaccgacgc
     6601 tgaggatatt gtgcaggaga ccctacttgc ggtgcatttg aagcggcaga cctgggatga
     6661 acaggcgccg ctcagtccgt ggcttttcgc catcgctcgt cacaagctga tcgatgcgtt
     6721 gcggcggcgc ggccggcgaa tagagctgtc gatcgacgat ttcgccgagt ctttgccgag
     6781 tgccgacgcg ggatccgact cgattgtatc cgacgtcaag cgccacctcg aagagctgcc
     6841 gcctggccag cgcaaggtgg tgaactgcat cgcagtcgag ggcgtctcga tcgacgaggc
     6901 ggcgagccgc ctgtcgatga gcaatggcgc cgttcgcgtc gctttgcatc ggggccttgc
     6961 ggcccttgcg gcaaaattca ggagtgttga atcatgaaga cgagcgagct tatcaacgcg
     7021 atttccgagg acagggagat gcccctggcc cctggccgcg cgttgtgcct cgcgctgatt
     7081 ccaggcctcg ctgtcgcgat cgcgctgttc tcgcgtttcg gcccgcggcc gcatttcctg
     7141 tcgttgctcg ccgacccgcg cttttccctg aagctgctgg tttgctatct gctcgcggcg
     7201 ctcgccggcc ttttcgtcct tcgcctgttc cgtccgggcg ggtcgtggcg gggcgctctg
     7261 tttctgctgg cgctgccgcc ggcgctgctt ggggtcggcg ttatcttcga attattcgtc
     7321 gtcagcccgg ccaattggga aacgacgatg atcggacaca acgccatgtt ctgcctgaag
     7381 actattccgc tccttggcat cgcgccgctc atcgccatcc tcgtggcgtt gcgcaacggc
     7441 gcgccggagc gcccggccgt cgccggcgcg ggcgctggcc tgttcgccgg ggcgatcggc
     7501 gcggcgctct acgcctccca ttgccccgat gattcgccgc tgttcgtggc ggtctggtat
     7561 ccgatcgcga tcgctttcct gacggcgctc ggcgcggcgg tcggggcgcg tcttttgcgc
     7621 tggtaaagcg tcacggcgac atgtcgccgc gcaaatcctt gcgcaggatc ttgccgacat
     7681 tcgatttcgg cagatcgtcg cggaagacga cgtgtcttgg tattttgtaa ggggcgagcc
     7741 gcgtccggca gaagtcgcgc aactcctcgg cgtcgagcga tttgtcggcg cggacgataa
     7801 aagccctgac cgcctcgccg acgccggcgt cgggcacgcc gaccaccgcg cattccttga
     7861 cgcccggatg ttcggtcagc gtcgcctcga cctcatttgg atagacattg aagccattga
     7921 cgaggatcat gtccttgcgc cggtcgacga gggtgaaata gccttcctca tccatggtgg
     7981 cgatgtcgcc ggtgtagagc cagccatcgc gcagggcgat cctggtttcc tcgggctggc
     8041 gccaatagcc gctcatgatc tgcggaccgc gcgcgatcag ttcgccatgt tcccccggcg
     8101 cgacttcgcg gccattgtcg tcgacgcatt tgatgtcggt ccagggcagc ggcacgccga
     8161 tcgttcccgg tttgacgcga agcgtgggat tgaaggtcag cacaggcgag gcttcgctga
     8221 ggccatagcc ctcgatcagc ggcgtctgcg tcacctgctc ccaccttttg gcgacgctct
     8281 cgtgcagcgc catgccgccg gcgacggaca ggcgcaaatg ctgtggcggc ttctcgacga
     8341 accagcgctc atgcagcagt cccttgaaca gggtgttgac gccggtaatg aaagaaatcg
     8401 gcgccttggc gaaggcgggc cgcagattaa cgagcgggcg cggattgggg atcagcacat
     8461 tgtgcgcgcc gcggaagaag aagcctagca tattcacggt aaaggcgaag caatgataga
     8521 gcggcagcgc agtcagcaca tgatcgtcct cgcgaatggc gccgccgacg aagctcaaga
     8581 attgcgcgac attcacgatc agattgcgat ggctgagcat agcggctttg ctgacgccgg
     8641 tggtgccgcc ggtatattgc aggcaggcga ccgcatcggg atcgagcccg gccgtatagg
     8701 cccgcacatc cgcgccttcc gagatttttt gcgcccccat ggcgagcgcc tgagcgaagg
     8761 cgcaggcggc aaagcgcggg cgagggattt cctttcggac atatttttgc acggccccga
     8821 tgatgagccg gggcgcggcc ggaaacaggc tcgccgccga ggtcaggaca acattgggaa
     8881 tgggcgcgtt ctccagcgca tccgccagtt tttccgcgaa catgtcgatg gcgacgatcg
     8941 cgtccggctg cgcatcggcg aacagatgca tcatttccgt ggcggtatag agcggattga
     9001 cgttgatgag cgtgcagccc gccttgaata tgccaaaggc gacgatcgga taggcgagcc
     9061 cgttcggggt ctgcaccgcg attcgcgccc ctgtcggaag tttcagattt tcgcgcagat
     9121 aggcggcgaa agcgtcggac agccggtcga cgtcattgaa ggagagccgc cccgacatgc
     9181 cattcggcat gactgtggtg aaggcgctcg ccccgccgcg cctcgccgcc agatcggcgg
     9241 gggcttcggc aagcagccgg tagcgcggcg tctcgatttc ggcgggaacg ctcgaggggt
     9301 aggtcttgag ccacggcggg cgcaaatcca cggcctgttc tctcatggcg tctgcccatt
     9361 gccccgcggg ttcttcgttt cttgtttcgt gtcgcgggcc aaagccgagc ccggagcgcg
     9421 gccgcggcct tgctccgcgc gccgctatga tatactcgcg gggcctattt gcctatcttg
     9481 gcgcgccgcg gcgcgcgagg cggtggcgac gccgcatgca ggagaaatcg cgacttggct
     9541 cagattccgg ctcagaattc gccttccccg gcgaatttgg cgacccccaa gccaatcaat
     9601 tggctgcatc tttcaaccat cgtcgcggtc gccatcctcg tcggcacgga aatggtcggc
     9661 gcgtcctggg cggcgggatg ggcgatcggc ggcctgttgc agctcgatcc ctcgatcagc
     9721 cggctgatcg agatcggctt caccctttgc ggcttcgtcc tcctctattt tttcatgcgc
     9781 atcgccatca ggcatgagcc gatccgcggc tgagccctac gcccgacgcc ccgacgcgct
     9841 gcgcgcatct catcacaagg gtttgagaat gactggcttt gaccggaggt cggtgctgaa
     9901 ggccgccgct gcgatgggtt ttagcgcggc gggattcagc gcggccgcgg ccttggaggg
     9961 gctgaagctc ggtccggcgc ggcctttctc ctttgaaggg ttgaaggcgg cggcaaagcg
    10021 cagggcggcc gagccctatg tcgggccgcc gcatccggcg cccgagatcg tcggcaagat
    10081 caattatgag gcctggggca aaatcgcctt cgacatggat catgcgctat tcgccaacgg
    10141 tccggggcgg tttccggtca gctttttcca tctcggcatg ttcttccaga aagccgtcga
    10201 gatgaatgtc gtcgaaggcg ccagcgcgcg cgagatcatc tacgatcaaa gctatttcac
    10261 gatgccggat gattccgtcg ccaaaaagct gccgcagggc gcgggctttg ccggcctgcg
    10321 tattcaggag ccgcgcgacg gcgcgctcga ctggcgcaag aatgactggg tcgcctttct
    10381 gggcgcggcc tatttccgtt cgatcggcgc gttgcgccaa tatggtctct ccgcccgagc
    10441 ggtggcgctt aacgtggccg tcgccggcaa gccggaagaa tttcccgatt tcaccaaatt
    10501 ctatatcgac gggtcggcga caggggattc gctgacgatc tatgccctgc ttgaagggcc
    10561 ctcgatcgtc ggcgcctgcc gtttcgtcat gacgcgcggc aaaggcgtcg tcatggacat
    10621 cgatcagacg ctgttcctgc gcgctgacgt gacgcggttc ggcctggcgc cggtcacctc
    10681 catgtactgg ttttccgaga cgaagaagga gaccggaatc gactggcgcc ctgaggtcca
    10741 tgattccgac gggctcgcca tctggtcggg gaccggcgag cggatctggc gtccgctgaa
    10801 caatccgccg cgcatcatgg cgtcggcttt cggcgacgaa aatccccgcg gcttcgggct
    10861 tttgcagcgc gatcgcaatt tcgatcatta t
//
